551-1415-00L  Image-based Drug Screening in Human Blood for Personalized Medicine

SemesterAutumn Semester 2020
LecturersB. Snijder, further lecturers
Periodicityyearly recurring course
Language of instructionEnglish
CommentNumber of participants limited to 5.
The enrolment is done by the D-BIOL study administration.

General safety regulations for all block courses:
-Whenever possible the distance rules have to be respected
-All students have to wear masks throughout the course (keep reserve masks ready)
-The installation and activation of the Swiss Covid-App is highly encouraged
-Any additional rules for individual courses have to be respected
-Students showing any COVID-19 symptoms are not allowed to enter ETH buildings and have to inform the course responsible


AbstractImage based screening allows to measure in high throughput the phenotype of millions of individual cells to external perturbations. We have recently shown that image-based screening in human blood can help to find active treatments for patients with blood cancers. In this course we will take the students through the entire workflow (to the extent that biosafety regulations allow it).
ObjectiveTake the students through the entire workflow from experimental design, to screen, to imaging and analysis.
-Learn to design an image-based screening experiment
-Observe human blood sample handling
-Perform immunofluorescence & automated confocal microscopy
-Image analysis and result interpretation
-Result presentation
Literature-Relevant study: https://www.thelancet.com/journals/lanhae/article/PIIS2352-3026(17)30208-9/fulltext
-Editorial commentary: https://www.thelancet.com/journals/lanhae/article/PIIS2352-3026(17)30213-2/fulltext